Data on variation in the chloroplast genome can be used to estimate the phylogeny of wheat. A specific region of cpDNA (~1600 base pairs) of wheat was amplified by PCR technique and this region was digested by several restriction enzymes such as EcoR I, Bcl I, Hsp92 I, Taq I, Hinf I and EcoR V. The banding patterns were compared by using agarose gel electrophoresis. After evaluation of the results, it has seen that the region which we studied on is very suitable for detection of interspecific and intraspecific variations between wild wheat species.