Biotechnology & Biotechnological Equipment

Subject: Biotechnology
Publisher: Diagnosis Press
ISSN 1310-2818

Volume 17, Number 2
Date: 2003

In vitro induction of crown galls by Agrobacterium tumefaciens super virulent strain 281 (pTiBo 54) in Salvia sclarea and S. pratense

K. M. Khawar¹, T. Unver¹ and E. Arican²

(1)	University of Ankara, Faculty of Agriculture, Department of Field Crops, 06110, Diskapi, Ankara, Turkey
(2)	Istanbul University, Science Faculty, Biology Department, Molecular Biology Section, 34459, Vezneciler/Istanbul, Turkey

Abstract

Salvia sclareae and S. pratense are important medicinal plants. The application of genetic engineering to these important crops should allow incorporation of some traits like insect and herbicide resistance. Co-cultivation experiments with A281 strain of Agrobacterium tumefaciens using leaf explants excised from 35 days old seedlings of both species showed that these were highly susceptible to transformation. Moreover, the strain A281 resulted in development of shooty tumors in S. sclarea and rooty tumors in S. pratense, which were selected on MS medium containing 50 mg/1 kanamycin. All of them were GUS positive.

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